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?coli S30 cell-free transcription-translation method regarding straight line templates (Promega). PCR-generated Genetics theme (0.1�C0.5 pmol) holding the actual required gene within the power over the Ptac ally had been translated within a 5?��l impulse containing 2?��Ci [35S]-methionine (distinct task 1,175 Ci/mmol) following a manufacturer��s standard protocol. Soon after 30�C45?min incubation

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